A novel approach to locate Phytophthora infestans resistance genes on the potato genetic map.
Identifieur interne : 001999 ( Main/Exploration ); précédent : 001998; suivant : 001A00A novel approach to locate Phytophthora infestans resistance genes on the potato genetic map.
Auteurs : Mirjam M J. Jacobs [Pays-Bas] ; Ben Vosman ; Vivianne G A A. Vleeshouwers ; Richard G F. Visser ; Betty Henken ; Ronald G. Van Den BergSource :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [ 1432-2242 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Maladies des plantes, Solanum tuberosum.
- immunologie : Maladies des plantes, Solanum tuberosum.
- microbiologie : Maladies des plantes, Solanum tuberosum.
- Cartographie chromosomique, Immunité innée, Phytophthora infestans.
English descriptors
- KwdEn :
- MESH :
- genetics : Plant Diseases, Solanum tuberosum.
- immunology : Plant Diseases, Solanum tuberosum.
- microbiology : Plant Diseases, Solanum tuberosum.
- Chromosome Mapping, Immunity, Innate, Phytophthora infestans.
Abstract
Mapping resistance genes is usually accomplished by phenotyping a segregating population for the resistance trait and genotyping it using a large number of markers. Most resistance genes are of the NBS-LRR type, of which an increasing number is sequenced. These genes and their analogs (RGAs) are often organized in clusters. Clusters tend to be rather homogenous, viz. containing genes that show high sequence similarity with each other. From many of these clusters the map position is known. In this study we present and test a novel method to quickly identify to which cluster a new resistance gene belongs and to produce markers that can be used for introgression breeding. We used NBS profiling to identify markers in bulked DNA samples prepared from resistant and susceptible genotypes of small segregating populations. Markers co-segregating with resistance can be tested on individual plants and directly used for breeding. To identify the resistance gene cluster a gene belongs to, the fragments were sequenced and the sequences analyzed using bioinformatics tools. Putative map positions arising from this analysis were validated using markers mapped in the segregating population. The versatility of the approach is demonstrated with a number of populations derived from wild Solanum species segregating for P. infestans resistance. Newly identified P. infestans resistance genes originating from S. verrucosum, S. schenckii, and S. capsicibaccatum could be mapped to potato chromosomes 6, 4, and 11, respectively.
DOI: 10.1007/s00122-009-1199-7
PubMed: 19902171
PubMed Central: PMC2812419
Affiliations:
Links toward previous steps (curation, corpus...)
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Jacobs</name><affiliation wicri:level="1"><nlm:affiliation>Biosystematics Group, Wageningen University and Research Centre, Generaal Foulkesweg 37, Wageningen, The Netherlands. m.jacobs@senternovem.nl</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Biosystematics Group, Wageningen University and Research Centre, Generaal Foulkesweg 37, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Vosman, Ben" sort="Vosman, Ben" uniqKey="Vosman B" first="Ben" last="Vosman">Ben Vosman</name></author><author><name sortKey="Vleeshouwers, Vivianne G A A" sort="Vleeshouwers, Vivianne G A A" uniqKey="Vleeshouwers V" first="Vivianne G A A" last="Vleeshouwers">Vivianne G A A. Vleeshouwers</name></author><author><name sortKey="Visser, Richard G F" sort="Visser, Richard G F" uniqKey="Visser R" first="Richard G F" last="Visser">Richard G F. Visser</name></author><author><name sortKey="Henken, Betty" sort="Henken, Betty" uniqKey="Henken B" first="Betty" last="Henken">Betty Henken</name></author><author><name sortKey="Van Den Berg, Ronald G" sort="Van Den Berg, Ronald G" uniqKey="Van Den Berg R" first="Ronald G" last="Van Den Berg">Ronald G. Van Den Berg</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19902171</idno><idno type="pmid">19902171</idno><idno type="doi">10.1007/s00122-009-1199-7</idno><idno type="pmc">PMC2812419</idno><idno type="wicri:Area/Main/Corpus">001996</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001996</idno><idno type="wicri:Area/Main/Curation">001996</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001996</idno><idno type="wicri:Area/Main/Exploration">001996</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A novel approach to locate Phytophthora infestans resistance genes on the potato genetic map.</title><author><name sortKey="Jacobs, Mirjam M J" sort="Jacobs, Mirjam M J" uniqKey="Jacobs M" first="Mirjam M J" last="Jacobs">Mirjam M J. Jacobs</name><affiliation wicri:level="1"><nlm:affiliation>Biosystematics Group, Wageningen University and Research Centre, Generaal Foulkesweg 37, Wageningen, The Netherlands. m.jacobs@senternovem.nl</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Biosystematics Group, Wageningen University and Research Centre, Generaal Foulkesweg 37, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Vosman, Ben" sort="Vosman, Ben" uniqKey="Vosman B" first="Ben" last="Vosman">Ben Vosman</name></author><author><name sortKey="Vleeshouwers, Vivianne G A A" sort="Vleeshouwers, Vivianne G A A" uniqKey="Vleeshouwers V" first="Vivianne G A A" last="Vleeshouwers">Vivianne G A A. Vleeshouwers</name></author><author><name sortKey="Visser, Richard G F" sort="Visser, Richard G F" uniqKey="Visser R" first="Richard G F" last="Visser">Richard G F. Visser</name></author><author><name sortKey="Henken, Betty" sort="Henken, Betty" uniqKey="Henken B" first="Betty" last="Henken">Betty Henken</name></author><author><name sortKey="Van Den Berg, Ronald G" sort="Van Den Berg, Ronald G" uniqKey="Van Den Berg R" first="Ronald G" last="Van Den Berg">Ronald G. Van Den Berg</name></author></analytic><series><title level="j">TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</title><idno type="eISSN">1432-2242</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosome Mapping (MeSH)</term><term>Immunity, Innate (MeSH)</term><term>Phytophthora infestans (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (immunology)</term><term>Solanum tuberosum (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cartographie chromosomique (MeSH)</term><term>Immunité innée (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (immunologie)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora infestans (MeSH)</term><term>Solanum tuberosum (génétique)</term><term>Solanum tuberosum (immunologie)</term><term>Solanum tuberosum (microbiologie)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term><term>Immunity, Innate</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie chromosomique</term><term>Immunité innée</term><term>Phytophthora infestans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mapping resistance genes is usually accomplished by phenotyping a segregating population for the resistance trait and genotyping it using a large number of markers. Most resistance genes are of the NBS-LRR type, of which an increasing number is sequenced. These genes and their analogs (RGAs) are often organized in clusters. Clusters tend to be rather homogenous, viz. containing genes that show high sequence similarity with each other. From many of these clusters the map position is known. In this study we present and test a novel method to quickly identify to which cluster a new resistance gene belongs and to produce markers that can be used for introgression breeding. We used NBS profiling to identify markers in bulked DNA samples prepared from resistant and susceptible genotypes of small segregating populations. Markers co-segregating with resistance can be tested on individual plants and directly used for breeding. To identify the resistance gene cluster a gene belongs to, the fragments were sequenced and the sequences analyzed using bioinformatics tools. Putative map positions arising from this analysis were validated using markers mapped in the segregating population. The versatility of the approach is demonstrated with a number of populations derived from wild Solanum species segregating for P. infestans resistance. Newly identified P. infestans resistance genes originating from S. verrucosum, S. schenckii, and S. capsicibaccatum could be mapped to potato chromosomes 6, 4, and 11, respectively.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19902171</PMID><DateCompleted><Year>2010</Year><Month>10</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2242</ISSN><JournalIssue CitedMedium="Internet"><Volume>120</Volume><Issue>4</Issue><PubDate><Year>2010</Year><Month>Feb</Month></PubDate></JournalIssue><Title>TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</Title><ISOAbbreviation>Theor Appl Genet</ISOAbbreviation></Journal><ArticleTitle>A novel approach to locate Phytophthora infestans resistance genes on the potato genetic map.</ArticleTitle><Pagination><MedlinePgn>785-96</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00122-009-1199-7</ELocationID><Abstract><AbstractText>Mapping resistance genes is usually accomplished by phenotyping a segregating population for the resistance trait and genotyping it using a large number of markers. Most resistance genes are of the NBS-LRR type, of which an increasing number is sequenced. These genes and their analogs (RGAs) are often organized in clusters. Clusters tend to be rather homogenous, viz. containing genes that show high sequence similarity with each other. From many of these clusters the map position is known. In this study we present and test a novel method to quickly identify to which cluster a new resistance gene belongs and to produce markers that can be used for introgression breeding. We used NBS profiling to identify markers in bulked DNA samples prepared from resistant and susceptible genotypes of small segregating populations. Markers co-segregating with resistance can be tested on individual plants and directly used for breeding. To identify the resistance gene cluster a gene belongs to, the fragments were sequenced and the sequences analyzed using bioinformatics tools. Putative map positions arising from this analysis were validated using markers mapped in the segregating population. The versatility of the approach is demonstrated with a number of populations derived from wild Solanum species segregating for P. infestans resistance. Newly identified P. infestans resistance genes originating from S. verrucosum, S. schenckii, and S. capsicibaccatum could be mapped to potato chromosomes 6, 4, and 11, respectively.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jacobs</LastName><ForeName>Mirjam M J</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Biosystematics Group, Wageningen University and Research Centre, Generaal Foulkesweg 37, Wageningen, The Netherlands. m.jacobs@senternovem.nl</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vosman</LastName><ForeName>Ben</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Vleeshouwers</LastName><ForeName>Vivianne G A A</ForeName><Initials>VG</Initials></Author><Author ValidYN="Y"><LastName>Visser</LastName><ForeName>Richard G F</ForeName><Initials>RG</Initials></Author><Author ValidYN="Y"><LastName>Henken</LastName><ForeName>Betty</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>van den Berg</LastName><ForeName>Ronald G</ForeName><Initials>RG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Theor Appl Genet</MedlineTA><NlmUniqueID>0145600</NlmUniqueID><ISSNLinking>0040-5752</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="Y">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="Y">Phytophthora 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sortKey="Van Den Berg, Ronald G" sort="Van Den Berg, Ronald G" uniqKey="Van Den Berg R" first="Ronald G" last="Van Den Berg">Ronald G. Van Den Berg</name><name sortKey="Visser, Richard G F" sort="Visser, Richard G F" uniqKey="Visser R" first="Richard G F" last="Visser">Richard G F. Visser</name><name sortKey="Vleeshouwers, Vivianne G A A" sort="Vleeshouwers, Vivianne G A A" uniqKey="Vleeshouwers V" first="Vivianne G A A" last="Vleeshouwers">Vivianne G A A. Vleeshouwers</name><name sortKey="Vosman, Ben" sort="Vosman, Ben" uniqKey="Vosman B" first="Ben" last="Vosman">Ben Vosman</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Jacobs, Mirjam M J" sort="Jacobs, Mirjam M J" uniqKey="Jacobs M" first="Mirjam M J" last="Jacobs">Mirjam M J. Jacobs</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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